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How to analyze germination of species with empty seeds using contemporary statistical methods? Acta Botanica
Santana,Denise Garcia de; Carvalho,Fábio Janoni; Toorop,Peter.
ABSTRACT Statistical analysis is considered an important tool for scientific studies, including those on seeds. However, seed scientists and statisticians often disagree on the nature of variables addressed in germination experiments. Statisticians consider the number of germinated seeds to be a binomially distributed variable, whereas seed scientists convert it into a percentage and often analyze it as a normally distributed variable. The requirement for normal adjustment restricts the models of analysis of variance that can be used. Lack of fit requires nonparametric tests, but they are known by their inferential problems. Generalized Linear Models (GLM) can provide better fit to germination variables for any species, including Lychnophora ericoides...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Brazilian arnica; Campos rupestres; Data transformation; Deviance analysis; Generalized Linear Models; Relative germination.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062018000200271
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Protein expression upon desiccation and imbibition of Magnolia ovata A. St.-Hil seeds BABT
José,Anderson Cleiton; Silva,Edvaldo Aparecido Amaral da; Davide,Antonio Claudio; Toorop,Peter.
The effect of seed drying and imbibition was studied by differential protein expression using two dimensional gel electrophoresis. After drying to a range of water contents, seeds were germinated to assess the viability. Seeds of Magnolia ovata did not withstand the desiccation down to 0.10 g H2O · g-1 dw. The critical water content below which the desiccation sensitivity became apparent was around 0.18 g H2O · g-1 dw (-26.5 MPa). Total protein was extracted and separated by 2D electrophoresis from fresh seeds (0.28 g H2O · g-1 dw), mild dried seeds (0.25 g H2O · g-1 dw) and seeds at low water content (0.10 g H2O · g-1 dw) before and after imbibition for 10 days. The proteome profile revealed the presence of 588 spots on each silver stained gel, from which...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Desiccation tolerance; Forest seed; Legumin; Proteomics.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000300006
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